prb s807 811 (Cell Signaling Technology Inc)

Cell Signaling Technology Inc is a verified supplier

Cell Signaling Technology Inc manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Cell Signaling Technology Inc prb s807 811
Prb S807 811, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 707 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prb+s807+811/Phospho-Rb+(Ser807%2F811)+XP+Rabbit+mAb/us12594421-308-38-41
Average 96 stars, based on 707 article reviews

prb s807 811 - by Bioz Stars, 2026-09

96/100 stars

Images

Electrophoresis:

Article Title: Compositions and methods for increasing cancer cell sensitivity to alternating electric fields
Article Snippet: Samples were run on SDS-polyacrylamide gel electrophoresis (Bolt 8% Bis-Tris base gel NW00080BOX, Thermo-Fischer). .. After electrophoresis, proteins were transferred to 0.2 μm polyvinylidene difluoride membrane (Immuno-Blot PVDF #162-0177, Bio-Rad) and probed with the appropriate primary antibody: GAPDH (SC-32233, Santa Cruz), Vinculin (ab140007, Abcam), Cyclin D1 (2922, Cell Signaling), Rb (9309, Cell Signaling), pRb S807/811 (8516, Cell Signaling), pAkt 5473, (4051, Cell signaling), Akt (2920, Cell signaling)), FAK (3283S, Cell signaling), Phospho-FAK (Tyr397) (3283S, Cell signaling, Src (2109S, cell signaling) and Phospho-Src (Tyr416) (2101S, Cell signaling). followed by horseradish peroxidase-conjugated secondary antibody (goat anti rabbit 7074, Cell Signaling and goat anti mouse 7076, Cell Signaling) and a chemiluminescent substrate (WBLUF0100, Signa-Aldrich). ..

Membrane:

other:

Article Title: Reversible proliferative arrest induced by rapid depletion of RNase MRP
Article Snippet: RPP40 (Proteintech, 11736-1-AP), POP1 (Abcam, ab254978), RPP21 (Proteintech, 16377-1-AP), RPP14 (Invitrogen, PA5-57567), RPP25 (Sigma, HPA046900-100UL), SLBP (Invitrogen, PA5-66410), Histone H3 (Abcam, ab1791), FLAG M2 (Sigma, F1804-200UG), Puromycin (Millipore, MABE343), Cyclin A2 (Cell Signaling Technology,4656), pRb S807/811 (Cell Signaling Technology, 9308), Rb (BD Biosciences, 554136), P21(Cell Signaling Technology, 2947), PCNA(Cell Signaling Technology, 13110), CDC6 (Abcam, ab188423), and beta Actin (Sigma, A3854) were also used in this study.

Western Blot:

Article Title: Intrinsic RB activation induces tumoral and stromal anti-tumor responses that limit triple-negative breast cancer.
Article Snippet: .. Primary antibodies for western blotting analysis included RB (4H1) (Cell Signaling, 9309L), pRB (S807/811) (Cell Signaling, 8516S), Cyclin A1 (Sigma, C4710), β-Actin (R&D Systems,MAB8929), and Flag (Proteintech, 20543-1-AP). .. The cells were lysed in RIPA lysis solution with 1X protease inhibitor (Thermo Fisher, 1860932) and 1mM Phenylmethanesulfonylfluoride (PMSF) (Sigma) to create whole-cell extracts.

Article Title: Intrinsic RB activation induces tumoral and stromal anti-tumor responses that limit triple-negative breast cancer
Article Snippet: .. Primary antibodies for western blotting analysis included RB (4H1) (Cell Signaling, 9309L), pRB (S807/811) (Cell Signaling, 8516S), Cyclin A1 (Sigma, C4710), β-Actin (R&D Systems, MAB8929), and Flag (Proteintech, 20543-1-AP). .. The cells were lysed in RIPA lysis solution with 1X protease inhibitor (Thermo Fisher, 1860932) and 1 mM Phenylmethanesulfonylfluoride (PMSF) (Sigma) to create whole-cell extracts.

Real-time Polymerase Chain Reaction:

Article Title: Reversible proliferative arrest induced by rapid depletion of RNase MRP.
Article Snippet: POP5 (sc-23046), RPP38 (sc-398113), RPP30 (sc-81374), RPP20 (sc244043), RPP29 (sc-23048), and E2F1 (sc-251) antibodies were from Santa Cruz. .. RPP40 (Proteintech, 11736-1-AP), POP1 (Abcam, ab254978), RPP21 (Proteintech, 16377-1-AP), RPP14 (Invitrogen, PA5-57567), RPP25 (Sigma, HPA046900-100UL), SLBP (Invitrogen, PA5-66410), Histone H3 (Abcam, ab1791), FLAG M2 (Sigma, F1804-200UG), Puromycin (Millipore,MABE343), Cyclin A2 (Cell SignalingTechnology,4656), pRb S807/811 (Cell Signaling Technology, 9308), Rb (BD Biosciences, 554136), P21(Cell Signaling Technology, 2947), PCNA(Cell Signaling Technology, 13110), CDC6 (Abcam, ab188423), and beta Actin (Sigma, A3854)were also used in this study.Mouse anti-p53/pab122 andmouse anti-MDM2/2A10 antibodies were gifts from Yanping Zhang. qPCR analysis qPCR was performed as previously described104. .. Briefly, total RNA was extracted from samples using TRIzol Reagent (ThemoFisher, 15596018) and Direct-zol RNA Miniprep (Zymo Research, R2050) according to the manufacturer’s instructions. cDNA was prepared from equal amounts of RNA using PrimeScript RT Reagent Kit (Takara, RR037A) following the manufacturer’s instructions. qPCR was performed using PowerUp SYBR Green Master Mix (ThermoFisher, A25742) and the oligonucleotide primers listed in Supplementary Data 2 to amplify the cDNA on the CFX Connect Real-Time PCR Detection System, operated by the CFXMaestro (v2.2) software, at the annealing temperature of 63 °C.

Review